human arachidonic acid (aa) elisa kit  (Cayman Chemical)

Journal: Gene

Article Title: The role of C/EBPβ phosphorylation in modulating membrane phospholipids repairing in LPS-induced human lung/bronchial epithelial cells

doi: 10.1016/j.gene.2017.07.076

Figure Lengend Snippet: Verification of ALI model and measurement of relevant proteins. A. TNFα, IL-1β, and IL-10 in BEAS-2B cells were examined using ELISA 12 h, 24 h or 48 h after treatment of 10 mg/ml LPS. The vertical coordinate indicates the concentration of target protein, and the horizontal coordinate indicates the experiment group. B. PAF and AA contents in BEAS-2B cells were measured using ELISA 12 h, 24 h or 48 h after treatment of 10 mg/ml LPS. The vertical coordinate indicates the concentration of target protein, and the horizontal coordinate indicates the experiment group. C. CDK2, CCSP1 and PLA2 expression levels, as well as C/EBP β phosphorylation, were assessed by western blotting 12 h, 24 h or 48 h after treatment of 10 mg/ml LPS. Lower: image of target blots, upper: analysis on the optical density of three proteins, β actin serving as the reference, and for the phosphorylated protein, the total protein of itself serving as the reference. Data are means ± SD from at least three separate replicates.*, p < 0.05; **, p < 0.01. vs cell group.

Article Snippet: Total protein was extracted and quantified using the BCA method, and intracellular TNFα, IL-1β and IL-10 were measured using Human TNFα-ELISA kit, Human IL-1β-ELISA kit and Human IL-10 ELISA kit (Invitrogen).Experiments for the PAF and AA level were performed using the Human arachidonic acid (AA) ELISA Kit (MBS703581, Cayman) and Human Platelet Activating Factor (PAF) ELISA Kit (MBS701223) according to the manufacturer's instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Expressing, Phospho-proteomics, Western Blot

Journal: Gene

Article Title: The role of C/EBPβ phosphorylation in modulating membrane phospholipids repairing in LPS-induced human lung/bronchial epithelial cells

doi: 10.1016/j.gene.2017.07.076

Figure Lengend Snippet: Verifying the specificity of the pathway and examining membrane damage A. BEAS-2B cells were infected with the indicated lentivirus, and PLA2 expression was measured by western blotting. Upper: representative blots and lower: the optical density of the target band divided by the optical density of the β-actin band. B. BEAS-2B cells were infected with the indicated lentivirus, and treated with LPS 48 h later, and PAF and AA were measured using ELISA. Data are means ± SD from at least three separate replicates.*, p < 0.05; **, p < 0.01.

Article Snippet: Total protein was extracted and quantified using the BCA method, and intracellular TNFα, IL-1β and IL-10 were measured using Human TNFα-ELISA kit, Human IL-1β-ELISA kit and Human IL-10 ELISA kit (Invitrogen).Experiments for the PAF and AA level were performed using the Human arachidonic acid (AA) ELISA Kit (MBS703581, Cayman) and Human Platelet Activating Factor (PAF) ELISA Kit (MBS701223) according to the manufacturer's instructions.

Techniques: Membrane, Infection, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: BMC Complementary and Alternative Medicine

Article Title: Antiplatelet and antithrombotic effects of cordycepin-enriched WIB-801CE from Cordyceps militaris ex vivo, in vivo, and in vitro

doi: 10.1186/s12906-016-1463-8

Figure Lengend Snippet: Ex vivo effects of WIB-801CE on phosphorylation of cPLA 2 , PLC β3, PLC γ2 , release of AA, and activity of COX-1 and TXAS. a Ex vivo effects of WIB-801CE on collagen-induced phosphorylation of cPLA 2 , PLC β3 and PLC γ2 . Lane 1, unstimulated platelets; Lane 2, collagen (10 μg/mL); Lane 3, collagen (10 μg/mL) + WIB-801CE (200 mg/kg-BW); Lane 4, collagen (10 μg/mL) + WIB-801CE (400 mg/kg-BW). b Ex vivo effects of WIB-801CE on ADP-induced phosphorylation of cPLA 2 , PLC β3 and PLC γ2 . Lane 1, unstimulated platelets; Lane 2, ADP (5 μM); Lane 3, ADP (5 μM) + WIB-801CE (200 mg/kg-BW); Lane 4, ADP (5 μM) + WIB-801CE (400 mg/kg-BW). c Ex vivo effects of WIB-801CE on collagen-induced arachidonic acid release. d Ex vivo effects of WIB-801CE on ADP-induced arachidonic acid release. e Ex vivo effects of WIB-801CE on COX-1 activity f Ex vivo effects of WIB-801CE on collagen-induced TXAS activity. g Ex vivo effects of WIB-801CE on ADP-induced TXAS activity. Measurements were carried out as described in “ ” section. The data are expressed as the mean ± standard deviation ( n = 4). * p < 0.05 versus each agonist-stimulated platelets. NS, not significant versus the each agonist-stimulated platelets, # p < 0.05 versus the ADP-stimulated platelets in the presence of WIB-801CE 200 mg/kg-BW. 1) Δ (%) = [(agonist + WIB-801CE 200 mg/kg-BW) – agonist]/agonist × 100

Article Snippet: Arachidonic acid (AA) release ELISA kit was purchased from Cusabio Biotech Corporation (Wuhan, Hubei, China).

Techniques: Ex Vivo, Phospho-proteomics, Activity Assay, Standard Deviation